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KMID : 0381920100400030155
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Korean Journal of Microscopy
2010 Volume.40 No. 3 p.155 ~ p.162
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Microstructure Analysis of Rabbit and Chicken Femurs by Light Microscopy and Transmission Electron Microscopy
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Kim Chang-Yeon
Kim Eun-Kyung
Jeon Tae-Hoon
Nam Seung-Won
Kim Youn-Joong
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Abstract
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Bone is a hierarchically structured composite material which has been well studied by the materials engineering community because of its unique structure and mechanical properties. Bone is a laminated organic-inorganic composite composed of primarily hydroxyapatite, collagen and water. The main mineral that gives bone’s hardness is calcium phosphate, which is also known as hydroxyapatite. Light microscopy (LM) and transmission electron microscopy (TEM) were used to study the structure of femurs from chicken and rabbit. The elemental analysis was used to search variation in the distribution of calcium, potassium and oxygen in the femur. Current investigation focused on two structural scales: micro scale (arrangement of compact bone) and nano scale (collagen fibril and apatite crystals). At micro scale, distinct difference was found in microstructures of chicken femur and rabbit femur. At nano scale, we analyzed the shape and size of apatite crystals and the arrangement of collagen fibril. Consequently, femurs of chicken and rabbit had very similar chemical property and structures at nano scale despite of their different species.
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KEYWORD
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Chicken femur, Rabbit femur, Microstructure, Electron diffraction pattern, Hydroxyapatite
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